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rc dc assay kit  (Bio-Rad)


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    Structured Review

    Bio-Rad rc dc assay kit
    Rc Dc Assay Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1895 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/protein+assay/RC+DC+Protein+Assay+Kit+I/10__22175_slash_mmb__17760-71-11-15
    Average 96 stars, based on 1895 article reviews
    rc dc assay kit - by Bioz Stars, 2026-09
    96/100 stars

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    other:

    Article Title: Drying-induced structural transitions in amyloid-like potato protein fibrils.
    Article Snippet: Amyloid-like fibrils derived from plant proteins are increasingly investigated as sustainable, label-friendly building blocks for food-grade functional materials.. In this study, we clarified the structural transition of potato protein fibrils formed under combined acidic and thermal conditions and examined how dehydration routes govern their post-processing integrity.. Fibrillation was verified across the tested concentration range by ThT fluorescence and Congo Red binding, together with the development of β-sheet-rich assemblies and shifts in surface charge.

    Article Title: Trimming Galactose Side Chains of Arabinogalactan Proteins Alters Pectin and Hemicellulose Deposition in Secondary Cell Walls of Arabidopsis thaliana Floral Stem Internodes.
    Article Snippet: Shaping the cell wall composition and structure to meet the requirements of different tissues and developmental stages relies on multiple actors, including arabinogalactan proteins (AGP).. Although the specific role of these proteins in cell wall dynamics is still under debate, especially in events involving significant remodeling of the cell wall, their carbohydrate motif, type II arabinogalactan (AGII), seems to be crucial for their function.. This study aims to investigate the function of AGII, specifically the galactose residues of its side chains, in the structural organization of the cell wall during the cessation of elongation and the transition to secondary growth.

    Article Title: Hydrogen sulfide increases intracellular oxygen and inhibits the HIF response
    Article Snippet: In parallel, 4 μl cleared lysate per sample was used for protein assay with 1 ml Bradford reagent (Bio-Rad).

    Protein Concentration:

    Article Title: miR-369-3p Modulates LRRK2-Mediated Inflammation and Autophagy in RAW264.7 Macrophages.
    Article Snippet: Total proteins were isolated using T-PER Protein Extraction Reagent (Thermo Fisher Scientific, Waltham, MA, USA) enriched with the protease inhibitor cocktail (Sigma-Aldrich, St. Louis, MO, USA), whereas cytoplasmic and nuclear separations were obtained using NE-PER lysis buffers (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions. .. The protein concentration was determined using Bradford’s protein assay (Biorad Laboratories, Hercules, CA, USA). .. Samples were separated using 7.5% and 4–20% Mini-PROTEAN TGX Stain-Free Protein Gels (Biorad Laboratories, Hercules, CA, USA) and then transferred onto PVDF membranes (Biorad Laboratories, Hercules, CA, USA).

    Article Title: miR-369-3p Modulates LRRK2-Mediated Inflammation and Autophagy in RAW264.7 Macrophages
    Article Snippet: Total proteins were isolated using T-PER Protein Extraction Reagent (Thermo Fisher Scientific, Waltham, MA, USA) enriched with the protease inhibitor cocktail (Sigma-Aldrich, St. Louis, MO, USA), whereas cytoplasmic and nuclear separations were obtained using NE-PER lysis buffers (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions. .. The protein concentration was determined using Bradford’s protein assay (Biorad Laboratories, Hercules, CA, USA). .. Samples were separated using 7.5% and 4–20% Mini-PROTEAN TGX Stain-Free Protein Gels (Biorad Laboratories, Hercules, CA, USA) and then transferred onto PVDF membranes (Biorad Laboratories, Hercules, CA, USA).

    Centrifugation:

    Article Title: Microcephaly-associated protein WDR62 supports purine metabolism by interacting with co-chaperone BAG2.
    Article Snippet: Protein extractions, immunoblots and coimmunoprecipitation After treatments, cells were lysed with RIPA buffer [150 mM NaCl, 100 mM Na3VO4, 50 mM Tris-HCl pH 7.3, 0.1 mM EDTA, 1% v/v Triton X-100, 1% w/v sodium deoxycholate and 0.2% w/v NaF] supplemented with protease inhibitors (cOmpleteTM, Mini, EDTAfree Protease Inhibitor Cocktail, Roche, 11836170001). .. Protein lysates were cleared by centrifugation (16,000×g, 15 min) and protein concentrations determined by protein assay with Bradford dye reagent (Bio-Rad, 5000006). ..

    Article Title: Microcephaly-associated protein WDR62 supports purine metabolism by interacting with co-chaperone BAG2
    Article Snippet: After treatments, cells were lysed with RIPA buffer [150 mM NaCl, 100 mM Na 3 VO 4 , 50 mM Tris-HCl pH 7.3, 0.1 mM EDTA, 1% v/v Triton X-100, 1% w/v sodium deoxycholate and 0.2% w/v NaF] supplemented with protease inhibitors (cOmpleteTM, Mini, EDTA-free Protease Inhibitor Cocktail, Roche, 11836170001). .. Protein lysates were cleared by centrifugation (16,000× g , 15 min) and protein concentrations determined by protein assay with Bradford dye reagent (Bio-Rad, 5000006). ..

    Lysis:

    Article Title: KDM5A methylation modulates its genomic demethylase and transcriptional actions.
    Article Snippet: Cells were incubated on ice for 10 min and debris was separated by spinning at 3500 rpm in a microcentrifuge (Eppendorf 5424R) at 4oC. .. The lysis step was repeated one more time and the supernatants were pooled for protein assay using BioRad DC kit (BioRad, 5000111). .. Protein lysates were pre- cleared with 80 L of pre-equilibrated Protein G Plus Agarose Suspension (Millipore IP04-1.5 mL) in lysis buffer for 1.5 h on a rotator at 4oC.



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    MedChemExpress dynamin related protein 1 drp1 inhibitor mdivi 1
    USP18 aggravates cardiac I/R injury through regulation of mitochondria and inhibition of mitophagy. a Electron microscopy image showing mitophagy in USP18-cKO mouse hearts ( n= 5). Scale bar=3 μm. White arrowheads indicate sites of mitophagy. b Protein levels of PINK1, Parkin, ubiquitinated proteins (Ub), P62, and LC3II in mitochondria from heart tissue in USP18-cKO and WT mice 24 h after I/R injury ( n =4). c Electron microscopy image showing mitophagy in USP18-overexpres (OV) mouse hearts ( n =5). Scale bar=3 μm. White arrowheads indicate sites of mitophagy. d Protein levels of PINK1, Parkin, Ub, P62, and LC3II proteins in mitochondria from the heart tissue of USP18-OV mice 24 h after I/R injury ( n =4). Color shift in mitophagy dye (red) and lysosomal dye (green) in NRVMs showing mitophagy in NRVMs with USP18 siRNA transfection ( e ) or Ad-USP18 infection ( f ) and the quantitative mitophagy index in each group ( n= 5). Scale bar=9 μm. Protein levels of PINK1, Parkin, Ub, P62, and LC3II in mitochondria from NRVMs transfected with USP18 siRNA ( g ) or infected with Ad-USP18 ( h ). ⁎⁎ P <0.01, ⁎⁎⁎ P <0.001 ⁎⁎⁎⁎ P <0.0001. USP18. Ubiquitin-specific protease 18; I/R. Ischemia/reperfusion; WT. Wild-type; KO. Knockout; P62. Sequestosome 1; LC3. Microtubule-associated <t>protein</t> <t>1</t> light chain 3; VDAC. Voltage-dependent anion channel.
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    USP18 aggravates cardiac I/R injury through regulation of mitochondria and inhibition of mitophagy. a Electron microscopy image showing mitophagy in USP18-cKO mouse hearts ( n= 5). Scale bar=3 μm. White arrowheads indicate sites of mitophagy. b Protein levels of PINK1, Parkin, ubiquitinated proteins (Ub), P62, and LC3II in mitochondria from heart tissue in USP18-cKO and WT mice 24 h after I/R injury ( n =4). c Electron microscopy image showing mitophagy in USP18-overexpres (OV) mouse hearts ( n =5). Scale bar=3 μm. White arrowheads indicate sites of mitophagy. d Protein levels of PINK1, Parkin, Ub, P62, and LC3II proteins in mitochondria from the heart tissue of USP18-OV mice 24 h after I/R injury ( n =4). Color shift in mitophagy dye (red) and lysosomal dye (green) in NRVMs showing mitophagy in NRVMs with USP18 siRNA transfection ( e ) or Ad-USP18 infection ( f ) and the quantitative mitophagy index in each group ( n= 5). Scale bar=9 μm. Protein levels of PINK1, Parkin, Ub, P62, and LC3II in mitochondria from NRVMs transfected with USP18 siRNA ( g ) or infected with Ad-USP18 ( h ). ⁎⁎ P <0.01, ⁎⁎⁎ P <0.001 ⁎⁎⁎⁎ P <0.0001. USP18. Ubiquitin-specific protease 18; I/R. Ischemia/reperfusion; WT. Wild-type; KO. Knockout; P62. Sequestosome 1; LC3. Microtubule-associated protein 1 light chain 3; VDAC. Voltage-dependent anion channel.

    Journal: Military Medical Research

    Article Title: USP18 exacerbates myocardial I/R injury by inhibiting Parkin mitophagy through the deubiquitinase PTEN-L

    doi: 10.1016/j.mmr.2026.100004

    Figure Lengend Snippet: USP18 aggravates cardiac I/R injury through regulation of mitochondria and inhibition of mitophagy. a Electron microscopy image showing mitophagy in USP18-cKO mouse hearts ( n= 5). Scale bar=3 μm. White arrowheads indicate sites of mitophagy. b Protein levels of PINK1, Parkin, ubiquitinated proteins (Ub), P62, and LC3II in mitochondria from heart tissue in USP18-cKO and WT mice 24 h after I/R injury ( n =4). c Electron microscopy image showing mitophagy in USP18-overexpres (OV) mouse hearts ( n =5). Scale bar=3 μm. White arrowheads indicate sites of mitophagy. d Protein levels of PINK1, Parkin, Ub, P62, and LC3II proteins in mitochondria from the heart tissue of USP18-OV mice 24 h after I/R injury ( n =4). Color shift in mitophagy dye (red) and lysosomal dye (green) in NRVMs showing mitophagy in NRVMs with USP18 siRNA transfection ( e ) or Ad-USP18 infection ( f ) and the quantitative mitophagy index in each group ( n= 5). Scale bar=9 μm. Protein levels of PINK1, Parkin, Ub, P62, and LC3II in mitochondria from NRVMs transfected with USP18 siRNA ( g ) or infected with Ad-USP18 ( h ). ⁎⁎ P <0.01, ⁎⁎⁎ P <0.001 ⁎⁎⁎⁎ P <0.0001. USP18. Ubiquitin-specific protease 18; I/R. Ischemia/reperfusion; WT. Wild-type; KO. Knockout; P62. Sequestosome 1; LC3. Microtubule-associated protein 1 light chain 3; VDAC. Voltage-dependent anion channel.

    Article Snippet: To block mitophagy, the selective dynamin-related protein 1 (Drp1) inhibitor Mdivi-1 was used (50 μmol/L, MedChemExpress, USA).

    Techniques: Inhibition, Electron Microscopy, Transfection, Infection, Ubiquitin Proteomics, Knock-Out